Feasibility of predicting the evolution of cerebral gliomas based on study of microRNA expression levels in blood plasma and saliva

The management of cerebral glioma (CG) remains challenging. Recently, methods based on the study expression levels miRNAs in blood plasma have proven to be promising. volume tumor tissue is known correlate with increased microRNA-21 and -210. MicroRNA-15, -16, -34, -126 -342 are involved regulation proliferative potential, microRNA-128 metabolic activity. aim was evaluate extended protocol for assessing microRNA-15, -21, -126, -128, -210, saliva CG patients. Material Methods. group consisted 24 patients supratentorial (8 men 16 women aged 41 71 years, mean age: 56 years). control 30 volunteers. MicroRNA studied according StemLoop-RealTime protocol, using fluorescently labeled samples small miRNA U6 as a reference gene. Statistical analysis carried out nonparametric methods. Results. lack stabilization most probable progression poor prognosis related an increase -210 decrease at least four microRNA 15, -342. Stabilization high probability associated or microRNA210 levels, no more than three well expression. low one miRNA-15, below level, simultaneous miRNA-128 Conclusion. determination can important criteria CG.